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凯发668k8开户登入·娱乐

凯发668k8开户登入

【导读】近日,凯发668k8开户登入【<】【p】【>】【 】【 】【省】【教】【学】【考】【试】【院】【提】【示】【,】【考】【生】【对】【这】【些】【疑】【问】【应】【分】【外】【留】【意】【。】【不】【管】【哪】【种】【过】【错】【,】【都】【有】【也】【许】【影】【响】【考】【生】【的】【正】【常】【选】【取】【,】【主】【张】【考】【生】【对】【照】【自】【愿】【草】【表】【逐】【个】【批】【次】【、】【逐】【个】【院】【校】【和】【专】【业】【自】【愿】【细】【心】【核】【对】【,】【发】【现】【疑】【问】【及】【时】【纠】【正】【,】【避】【免】【影】【响】【选】【取】【。】【<】【/】【p】【>】【<】【t】【d】【 】【s】【t】【y】【l】【e】【=】【"】【p】【a】【d】【d】【i】【n】【g】【-】【b】【o】【t】【t】【o】【m】【:】【 】【0】【p】【x】【;】【 】【p】【a】【d】【d】【i】【n】【g】【-】【t】【o】【p】【:】【 】【0】【p】【x】【;】【 】【p】【a】【d】【d】【i】【n】【g】【-】【l】【e】【f】【t】【:】【 】【0】【p】【x】【;】【 】【m】【a】【r】【g】【i】【n】【:】【 】【0】【p】【x】【;】【 】【p】【a】【d】【d】【i】【n】【g】【-】【r】【i】【g】【h】【t】【:】【 】【0】【p】【x】【"】【>】【-】【<】【/】【t】【d】【>】【<】【p】【>】【 】【 】【(】【二】【)】【考】【生】【必】【须】【在】【填】【写】【截】【止】【时】【刻】【前】【完】【结】【网】【上】【填】【写】【自】【愿】【,】【并】【自】【觉】【在】【承】【认】【截】【止】【时】【刻】【前】【到】【承】【认】【点】【进】【行】【承】【认】【。】【抵】【达】【承】【认】【截】【止】【时】【刻】【时】【,】【承】【认】【点】【要】【完】【结】【数】【据】【提】【交】【作】【业】【。】【一】【经】【提】【交】【,】【任】【何】【人】【(】【包】【含】【考】【生】【自】【个】【)】【不】【得】【修】【正】【考】【生】【自】【愿】【信】【息】【。】【<】【/】【p】【>】

凯发668k8开户登入

20200410,凯发668k8开户登入登上热搜,20200410,【<】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【2】【0】【1】【7】【 】【年】【 】【6】【 】【月】【 】【1】【6】【 】【日】【,】【北】【京】【大】【学】【生】【命】【科】【学】【学】【院】【生】【物】【动】【态】【光】【学】【成】【像】【基】【地】【汤】【富】【酬】【课】【题】【组】【在】【《】【C】【e】【l】【l】【 】【R】【e】【s】【e】【a】【r】【c】【h】【》】【杂】【志】【在】【线】【宣】【布】【了】【题】【为】【 】【“】【S】【i】【n】【g】【l】【e】【-】【c】【e】【l】【l】【 】【m】【u】【l】【t】【i】【-】【o】【m】【i】【c】【s】【 】【s】【e】【q】【u】【e】【n】【c】【i】【n】【g】【 】【o】【f】【 】【m】【o】【u】【s】【e】【 】【e】【a】【r】【l】【y】【 】【e】【m】【b】【r】【y】【o】【s】【 】【a】【n】【d】【 】【e】【m】【b】【r】【y】【o】【n】【i】【c】【 】【s】【t】【e】【m】【 】【c】【e】【l】【l】【s】【”】【 】【的】【研】【讨】【论】【文】【。】【在】【国】【际】【上】【首】【先】【开】【展】【了】【对】【一】【个】【单】【细】【胞】【一】【起】【进】【行】【染】【色】【质】【状】【况】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【、】【以】【及】【染】【色】【体】【倍】【性】【的】【全】【基】【因】【组】【测】【序】【技】【能】【(】【s】【i】【n】【g】【l】【e】【-】【c】【e】【l】【l】【 】【C】【O】【O】【L】【-】【s】【e】【q】【)】【,】【并】【选】【用】【这】【一】【技】【能】【在】【单】【细】【胞】【分】【辨】【率】【上】【体】【系】【、】【深】【化】【地】【解】【析】【了】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【表】【观】【基】【因】【组】【重】【编】【程】【的】【要】【害】【特】【征】【,】【以】【及】【染】【色】【质】【状】【况】【与】【 】【D】【N】【A】【 】【甲】【基】【化】【之】【间】【的】【互】【动】【联】【系】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【现】【有】【的】【依】【据】【高】【通】【量】【测】【序】【来】【剖】【析】【全】【基】【因】【组】【染】【色】【质】【状】【况】【的】【研】【讨】【办】【法】【通】【常】【需】【求】【很】【多】【细】【胞】【(】【例】【如】【 】【A】【T】【A】【C】【-】【s】【e】【q】【、】【D】【N】【a】【s】【e】【-】【s】【e】【q】【、】【F】【A】【I】【R】【E】【-】【s】【e】【q】【、】【M】【N】【a】【s】【e】【-】【s】【e】【q】【 】【等】【)】【。】【即】【便】【这】【些】【办】【法】【能】【够】【做】【到】【单】【细】【胞】【分】【辨】【率】【,】【也】【无】【法】【对】【同】【一】【个】【单】【细】【胞】【的】【各】【个】【表】【观】【基】【因】【组】【层】【面】【一】【起】【进】【行】【剖】【析】【,】【因】【此】【无】【法】【在】【单】【细】【胞】【分】【辨】【率】【上】【对】【多】【种】【组】【学】【之】【间】【的】【互】【动】【联】【系】【进】【行】【研】【讨】【。】【而】【汤】【富】【酬】【课】【题】【组】【将】【 】【N】【O】【M】【e】【-】【s】【e】【q】【(】【全】【基】【因】【组】【核】【小】【体】【定】【位】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【组】【测】【序】【)】【技】【能】【和】【 】【P】【B】【A】【T】【-】【s】【e】【q】【 】【技】【能】【(】【全】【基】【因】【组】【重】【亚】【硫】【酸】【盐】【测】【序】【)】【奇】【妙】【地】【联】【系】【起】【来】【(】【图】【 】【1】【)】【,】【并】【进】【行】【了】【体】【系】【的】【优】【化】【和】【进】【步】【,】【完】【结】【了】【对】【同】【一】【个】【单】【细】【胞】【进】【行】【多】【达】【 】【5】【 】【个】【层】【面】【(】【染】【色】【质】【状】【况】【、】【核】【小】【体】【定】【位】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【和】【染】【色】【体】【倍】【性】【)】【的】【基】【因】【组】【和】【表】【观】【基】【因】【组】【特】【征】【的】【剖】【析】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【1】【:】【 】【s】【i】【n】【g】【l】【e】【-】【c】【e】【l】【l】【 】【C】【O】【O】【L】【-】【s】【e】【q】【 】【原】【理】【图】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【除】【了】【能】【够】【在】【单】【细】【胞】【水】【平】【上】【一】【起】【对】【多】【种】【组】【学】【进】【行】【剖】【析】【,】【与】【现】【有】【的】【剖】【析】【染】【色】【质】【状】【况】【的】【单】【细】【胞】【测】【序】【技】【能】【比】【较】【,】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【技】【能】【还】【具】【有】【如】【下】【长】【处】【:】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【1】【)】【关】【于】【基】【因】【组】【中】【基】【地】【的】【功】【用】【元】【件】【区】【域】【具】【有】【很】【高】【的】【灵】【敏】【度】【和】【掩】【盖】【度】【。】【例】【如】【,】【关】【于】【小】【鼠】【胚】【胎】【干】【细】【胞】【系】【的】【一】【个】【单】【细】【胞】【,】【能】【够】【一】【起】【检】【查】【到】【 】【1】【8】【,】【0】【0】【0】【 】【多】【个】【基】【因】【(】【一】【切】【已】【知】【基】【因】【的】【 】【7】【5】【%】【)】【的】【发】【动】【子】【区】【域】【的】【染】【色】【质】【状】【况】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【,】【 】【也】【能】【够】【一】【起】【检】【查】【到】【 】【1】【1】【,】【0】【0】【0】【 】【多】【个】【 】【C】【p】【G】【 】【岛】【(】【一】【切】【已】【知】【 】【C】【p】【G】【 】【岛】【的】【 】【7】【0】【%】【)】【的】【染】【色】【质】【状】【况】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【;】【这】【为】【剖】【析】【干】【细】【胞】【分】【解】【发】【育】【进】【程】【中】【染】【色】【质】【状】【况】【的】【异】【质】【性】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【的】【异】【质】【性】【供】【给】【了】【强】【壮】【的】【东】【西】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【2】【)】【能】【够】【精】【准】【地】【判】【定】【出】【染】【色】【质】【的】【敞】【开】【状】【况】【和】【封】【闭】【状】【况】【,】【精】【确】【地】【把】【染】【色】【质】【封】【闭】【状】【况】【与】【因】【为】【技】【能】【因】【素】【未】【检】【查】【到】【的】【状】【况】【(】【假】【阴】【性】【)】【区】【别】【开】【来】【。】【现】【有】【的】【别】【的】【单】【细】【胞】【染】【色】【质】【状】【况】【测】【序】【技】【能】【(】【例】【如】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【和】【 】【s】【c】【D】【N】【a】【s】【e】【-】【s】【e】【q】【 】【技】【能】【)】【无】【法】【将】【染】【色】【质】【封】【闭】【状】【况】【和】【未】【检】【查】【到】【的】【状】【况】【(】【假】【阴】【性】【)】【区】【别】【开】【,】【这】【一】【缺】【点】【在】【现】【有】【技】【能】【的】【灵】【敏】【度】【比】【较】【低】【的】【状】【况】【下】【尤】【为】【严】【峻】【。】【而】【 】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【技】【能】【能】【够】【精】【确】【地】【区】【别】【染】【色】【质】【封】【闭】【状】【况】【(】【检】【查】【到】【了】【该】【基】【因】【组】【 】【D】【N】【A】【 】【片】【段】【,】【可】【是】【其】【间】【的】【 】【G】【p】【C】【 】【位】【点】【没】【有】【被】【甲】【基】【化】【)】【和】【因】【为】【技】【能】【因】【素】【未】【检】【查】【到】【的】【状】【况】【(】【没】【有】【检】【查】【到】【该】【基】【因】【组】【 】【D】【N】【A】【 】【片】【段】【)】【。】【这】【么】【对】【一】【种】【细】【胞】【类】【型】【中】【不】【一】【样】【的】【单】【个】【细】【胞】【之】【间】【同】【一】【个】【基】【因】【组】【区】【域】【的】【染】【色】【质】【敞】【开】【状】【况】【和】【封】【闭】【状】【况】【之】【间】【的】【份】【额】【能】【够】【给】【出】【精】【确】【的】【丈】【量】【成】【果】【,】【而】【不】【受】【检】【查】【灵】【敏】【度】【动】【摇】【的】【影】【响】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【3】【)】【能】【够】【对】【同】【一】【个】【 】【D】【N】【A】【 】【单】【分】【子】【一】【起】【取】【得】【其】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【信】【息】【,】【使】【得】【这】【一】【技】【能】【不】【光】【具】【有】【单】【细】【胞】【分】【辨】【率】【,】【乃】【至】【到】【达】【了】【单】【分】【子】【分】【辨】【率】【,】【能】【够】【对】【同】【一】【个】【二】【倍】【体】【细】【胞】【的】【两】【个】【等】【位】【基】【因】【别】【离】【进】【行】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【剖】【析】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【4】【)】【因】【为】【该】【办】【法】【一】【起】【扩】【增】【和】【测】【序】【敞】【开】【的】【和】【封】【闭】【的】【染】【色】【质】【区】【域】【,】【因】【此】【不】【受】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【含】【量】【动】【摇】【的】【影】【响】【。】【在】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【通】【常】【是】【环】【形】【暴】【露】【的】【 】【D】【N】【A】【,】【通】【常】【处】【于】【敞】【开】【状】【况】【。】【现】【有】【的】【别】【的】【单】【细】【胞】【染】【色】【质】【状】【况】【测】【序】【技】【能】【(】【例】【如】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【和】【 】【s】【c】【D】【N】【a】【s】【e】【-】【s】【e】【q】【 】【技】【能】【)】【因】【为】【只】【扩】【增】【和】【测】【序】【敞】【开】【的】【染】【色】【质】【区】【域】【,】【因】【此】【极】【大】【地】【受】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【含】【量】【的】【影】【响】【,】【特】【别】【是】【在】【着】【床】【前】【的】【前】【期】【胚】【胎】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【的】【含】【量】【是】【一】【般】【细】【胞】【的】【数】【十】【倍】【乃】【至】【数】【百】【倍】【,】【因】【此】【关】【于】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【和】【 】【s】【c】【D】【N】【a】【s】【e】【-】【s】【e】【q】【 】【等】【技】【能】【形】【成】【严】【峻】【影】【响】【。】【而】【 】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【技】【能】【因】【为】【一】【起】【扩】【增】【和】【测】【序】【敞】【开】【的】【和】【封】【闭】【的】【染】【色】【质】【区】【域】【而】【不】【受】【这】【一】【疑】【问】【的】【影】【响】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【5】【)】【因】【为】【掺】【入】【了】【 】【1】【0】【%】【 】【-】【 】【2】【0】【%】【 】【的】【外】【源】【 】【l】【a】【m】【b】【d】【a】【 】【D】【N】【A】【,】【所】【以】【能】【够】【精】【确】【判】【别】【所】【剖】【析】【单】【个】【细】【胞】【所】【在】【的】【细】【胞】【周】【期】【期】【间】【以】【及】【染】【色】【体】【倍】【性】【。】【这】【关】【于】【精】【确】【剖】【析】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【与】【细】【胞】【周】【期】【以】【及】【染】【色】【体】【倍】【性】【的】【联】【系】【十】【分】【有】【协】【助】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【考】【虑】【到】【多】【组】【学】【的】【信】【息】【量】【、】【有】【用】【数】【据】【量】【带】【来】【的】【昂】【扬】【测】【序】【本】【钱】【等】【疑】【问】【,】【关】【于】【细】【胞】【数】【量】【少】【,】【异】【质】【性】【强】【的】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【的】【研】【讨】【,】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【办】【法】【十】【分】【合】【适】【。】【该】【办】【法】【能】【够】【非】【常】【好】【地】【掩】【盖】【全】【基】【因】【组】【,】【有】【用】【地】【处】【理】【了】【如】【今】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【研】【讨】【中】【线】【粒】【体】【片】【段】【过】【度】【富】【集】【致】【使】【的】【有】【用】【数】【据】【量】【过】【少】【的】【疑】【问】【。】【一】【起】【,】【该】【办】【法】【能】【够】【一】【起】【剖】【析】【单】【个】【细】【胞】【中】【染】【色】【质】【敞】【开】【程】【度】【、】【核】【小】【体】【定】【位】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【、】【以】【及】【染】【色】【体】【倍】【性】【这】【 】【5】【 】【个】【组】【学】【层】【面】【,】【关】【于】【难】【以】【很】【多】【取】【得】【的】【哺】【乳】【动】【物】【前】【期】【胚】【胎】【的】【发】【育】【、】【以】【及】【杂】【乱】【的】【癌】【症】【等】【疾】【病】【研】【讨】【,】【都】【将】【供】【给】【全】【部】【有】【用】【的】【处】【理】【方】【案】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【在】【此】【根】【底】【上】【,】【该】【课】【题】【组】【运】【用】【这】【一】【新】【树】【立】【的】【 】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【办】【法】【,】【在】【单】【细】【胞】【分】【辨】【率】【体】【系】【地】【描】【写】【了】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【表】【观】【基】【因】【组】【多】【个】【层】【面】【的】【动】【态】【改】【变】【。】【高】【度】【特】【化】【的】【精】【子】【和】【卵】【细】【胞】【联】【系】【后】【,】【会】【进】【行】【一】【系】【列】【杂】【乱】【、】【精】【确】【的】【染】【色】【质】【重】【塑】【进】【程】【,】【然】【后】【树】【立】【起】【前】【期】【胚】【胎】【发】【育】【的】【全】【能】【性】【和】【多】【能】【性】【。】【对】【该】【进】【程】【的】【解】【析】【将】【有】【利】【于】【大】【家】【了】【解】【细】【胞】【多】【能】【性】【树】【立】【进】【程】【中】【的】【表】【观】【遗】【传】【调】【控】【机】【制】【以】【及】【胚】【胎】【发】【育】【反】【常】【的】【分】【子】【机】【理】【,】【该】【项】【研】【讨】【发】【现】【:】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【1】【)】【受】【精】【后】【 】【1】【2】【 】【小】【时】【以】【内】【,】【来】【自】【高】【度】【特】【化】【的】【卵】【细】【胞】【和】【精】【子】【的】【男】【女】【原】【核】【就】【阅】【历】【了】【大】【规】【模】【的】【基】【因】【组】【去】【甲】【基】【化】【,】【父】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【程】【度】【从】【精】【子】【中】【的】【 】【8】【0】【%】【(】【平】【均】【数】【)】【降】【低】【到】【雄】【原】【核】【中】【的】【 】【3】【8】【%】【(】【p】【=】【1】【.】【4】【×】【1】【0】【-】【1】【1】【)】【;】【一】【起】【母】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【程】【度】【从】【卵】【细】【胞】【中】【的】【 】【3】【2】【%】【 】【降】【低】【到】【雌】【原】【核】【中】【的】【 】【2】【8】【%】【(】【p】【=】【6】【.】【3】【×】【1】【0】【-】【5】【)】【。】【在】【此】【进】【程】【中】【,】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【体】【状】【况】【敏】【捷】【翻】【开】【,】【在】【受】【精】【卵】【的】【原】【核】【期】【就】【现】【已】【到】【达】【高】【度】【敞】【开】【的】【状】【况】【,】【随】【后】【在】【受】【精】【卵】【晚】【期】【染】【色】【质】【敞】【开】【程】【度】【大】【幅】【度】【回】【落】【,】【并】【在】【 】【2】【 】【-】【 】【细】【胞】【期】【间】【以】【后】【敞】【开】【程】【度】【再】【次】【逐】【步】【添】【加】【,】【到】【囊】【胚】【期】【时】【到】【达】【最】【高】【点】【(】【图】【 】【2】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【2】【:】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【基】【因】【组】【内】【源】【 】【D】【N】【A】【 】【甲】【基】【化】【(】【A】【)】【与】【染】【色】【质】【状】【况】【(】【B】【)】【以】【及】【染】【色】【质】【状】【况】【异】【质】【性】【(】【基】【因】【发】【动】【子】【区】【域】【;】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【o】【p】【e】【n】【、】【D】【i】【v】【e】【r】【g】【e】【n】【t】【、】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【c】【l】【o】【s】【e】【d】【 】【三】【种】【异】【质】【性】【状】【况】【)】【(】【C】【)】【(】【D】【)】【的】【动】【态】【改】【变】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【2】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【体】【系】【剖】【析】【了】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【染】【色】【质】【状】【况】【的】【异】【质】【性】【。】【依】【据】【每】【个】【基】【因】【发】【动】【子】【区】【域】【在】【同】【一】【个】【发】【育】【期】【间】【不】【一】【样】【单】【细】【胞】【之】【间】【染】【色】【质】【状】【况】【的】【异】【质】【性】【,】【将】【小】【鼠】【的】【基】【因】【划】【分】【红】【均】【匀】【敞】【开】【(】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【o】【p】【e】【n】【)】【、】【敞】【开】【 】【/】【 】【封】【闭】【混】【合】【态】【(】【D】【i】【v】【e】【r】【g】【e】【n】【t】【)】【、】【均】【匀】【封】【闭】【(】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【c】【l】【o】【s】【e】【d】【)】【这】【三】【种】【状】【况】【。】【该】【研】【讨】【发】【如】【今】【受】【精】【后】【 】【1】【2】【 】【个】【小】【时】【以】【内】【受】【精】【卵】【中】【大】【有】【些】【基】【因】【的】【发】【动】【子】【区】【域】【就】【由】【均】【匀】【封】【闭】【状】【况】【敏】【捷】【重】【编】【程】【为】【均】【匀】【敞】【开】【状】【况】【,】【为】【合】【子】【基】【因】【在】【随】【后】【的】【转】【录】【做】【好】【预】【备】【(】【图】【 】【2】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【3】【)】【经】【过】【 】【R】【N】【A】【 】【聚】【合】【酶】【按】【捺】【剂】【按】【捺】【转】【录】【的】【试】【验】【,】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【证】【实】【持】【续】【转】【录】【关】【于】【保】【持】【基】【因】【发】【动】【子】【区】【域】【的】【染】【色】【质】【敞】【开】【状】【况】【具】【有】【主】【要】【作】【用】【。】【从】【受】【精】【前】【的】【卵】【细】【胞】【到】【受】【精】【后】【的】【晚】【期】【受】【精】【卵】【期】【间】【,】【发】【动】【子】【区】【大】【片】【段】【染】【色】【质】【敞】【开】【区】【域】【(】【长】【度】【大】【于】【 】【3】【0】【0】【b】【p】【)】【的】【数】【量】【大】【幅】【度】【添】【加】【。】【该】【研】【讨】【发】【现】【,】【运】【用】【 】【R】【N】【A】【 】【聚】【合】【酶】【按】【捺】【剂】【α】【-】【A】【m】【a】【n】【i】【t】【i】【n】【 】【处】【理】【受】【精】【卵】【、】【按】【捺】【其】【转】【录】【活】【动】【,】【会】【致】【使】【数】【千】【个】【(】【5】【6】【%】【)】【基】【因】【的】【发】【动】【子】【区】【大】【片】【段】【染】【色】【质】【敞】【开】【区】【域】【从】【头】【封】【闭】【,】【阐】【明】【持】【续】【的】【转】【录】【关】【于】【保】【持】【前】【期】【胚】【胎】【中】【大】【有】【些】【基】【因】【的】【发】【动】【子】【处】【于】【敞】【开】【状】【况】【是】【必】【需】【的】【,】【染】【色】【质】【状】【况】【敞】【开】【和】【转】【录】【活】【动】【互】【相】【促】【进】【,】【一】【起】【保】【持】【合】【子】【基】【因】【的】【安】【稳】【表】【达】【(】【图】【 】【3】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【4】【)】【发】【现】【多】【能】【性】【基】【地】【因】【子】【 】【O】【c】【t】【4】【 】【的】【靶】【基】【因】【联】【系】【位】【点】【在】【 】【4】【 】【-】【 】【细】【胞】【期】【间】【就】【现】【已】【翻】【开】【并】【处】【于】【敞】【开】【状】【况】【,】【远】【早】【于】【真】【实】【树】【立】【多】【能】【性】【的】【囊】【胚】【期】【,】【暗】【示】【这】【些】【位】【点】【作】【为】【潜】【在】【的】【顺】【式】【调】【控】【元】【件】【也】【许】【参】【加】【了】【前】【期】【胚】【胎】【细】【胞】【的】【命】【运】【决】【议】【进】【程】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【5】【)】【初】【次】【在】【单】【个】【细】【胞】【内】【对】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【进】【行】【了】【深】【化】【剖】【析】【。】【从】【受】【精】【卵】【晚】【期】【到】【 】【4】【 】【细】【胞】【胚】【胎】【期】【间】【,】【在】【基】【因】【间】【区】【(】【i】【n】【t】【e】【r】【g】【e】【n】【i】【c】【 】【r】【e】【g】【i】【o】【n】【)】【父】【源】【基】【因】【组】【甲】【基】【化】【要】【显】【着】【高】【于】【母】【源】【基】【因】【组】【;】【而】【在】【基】【因】【区】【(】【g】【e】【n】【e】【 】【b】【o】【d】【y】【)】【,】【父】【源】【基】【因】【组】【甲】【基】【化】【要】【显】【着】【低】【于】【母】【源】【基】【因】【组】【。】【这】【是】【因】【为】【在】【基】【因】【间】【区】【,】【父】【源】【基】【因】【组】【的】【去】【甲】【基】【化】【速】【度】【较】【慢】【;】【而】【在】【基】【因】【区】【,】【父】【源】【基】【因】【组】【的】【去】【甲】【基】【化】【速】【度】【要】【远】【快】【于】【母】【源】【基】【因】【组】【的】【去】【甲】【基】【化】【速】【度】【。】【更】【主】【要】【的】【是】【,】【关】【于】【基】【因】【区】【,】【胚】【胎】【期】【表】【达】【水】【平】【越】【高】【的】【基】【因】【其】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【不】【对】【称】【性】【越】【激】【烈】【(】【表】【达】【水】【平】【越】【高】【,】【母】【源】【基】【因】【组】【甲】【基】【化】【就】【比】【父】【源】【基】【因】【组】【高】【越】【多】【)】【。】【与】【此】【相】【反】【,】【受】【精】【后】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【就】【敏】【捷】【同】【步】【翻】【开】【,】【到】【受】【精】【后】【 】【1】【2】【 】【小】【时】【,】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【在】【每】【个】【单】【细】【胞】【中】【就】【现】【已】【到】【达】【一】【样】【的】【敞】【开】【状】【况】【,】【并】【在】【全】【部】【植】【入】【前】【期】【间】【保】【持】【这】【一】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【之】【间】【染】【色】【质】【状】【况】【的】【精】【确】【平】【衡】【。】【这】【阐】【明】【受】【精】【后】【,】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【进】【行】【了】【不】【一】【样】【步】【的】【重】【编】【程】【进】【程】【,】【因】【为】【染】【色】【质】【状】【况】【关】【于】【合】【子】【基】【因】【的】【激】【活】【也】【许】【更】【主】【要】【,】【所】【以】【受】【精】【后】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【敏】【捷】【重】【编】【程】【、】【在】【每】【个】【单】【细】【胞】【中】【敏】【捷】【到】【达】【精】【确】【平】【衡】【并】【一】【向】【保】【持】【。】【而】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【重】【编】【程】【要】【慢】【一】【些】【并】【在】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【之】【间】【保】【持】【不】【对】【称】【散】【布】【:】【在】【基】【因】【间】【区】【,】【父】【源】【基】【因】【组】【甲】【基】【化】【高】【于】【母】【源】【基】【因】【组】【;】【而】【在】【基】【因】【区】【,】【父】【源】【基】【因】【组】【甲】【基】【化】【低】【于】【母】【源】【基】【因】【组】【,】【而】【且】【与】【胚】【胎】【期】【基】【因】【表】【达】【水】【平】【有】【关】【(】【图】【 】【4】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【4】【:】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【不】【对】【称】【散】【布】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【6】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【解】【析】【了】【雌】【性】【胚】【胎】【细】【胞】【中】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【重】【编】【程】【进】【程】【的】【异】【同】【。】【从】【配】【子】【到】【原】【核】【期】【受】【精】【卵】【,】【父】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【去】【甲】【基】【化】【速】【度】【显】【着】【比】【常】【染】【色】【体】【慢】【,】【而】【母】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【去】【甲】【基】【化】【速】【度】【和】【常】【染】【色】【体】【适】【当】【。】【从】【受】【精】【卵】【晚】【期】【到】【 】【4】【 】【细】【胞】【胚】【胎】【期】【间】【,】【父】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【显】【着】【高】【于】【母】【源】【 】【X】【 】【染】【色】【体】【,】【直】【到】【囊】【胚】【期】【,】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【才】【到】【达】【一】【起】【。】【这】【阐】【明】【受】【精】【以】【后】【,】【在】【雌】【性】【胚】【胎】【中】【失】【活】【的】【父】【源】【 】【X】【 】【染】【色】【体】【其】【 】【D】【N】【A】【 】【甲】【基】【化】【重】【编】【程】【速】【度】【要】【显】【着】【慢】【于】【活】【泼】【的】【母】【源】【 】【X】【 】【染】【色】【体】【。】【二】【者】【之】【间】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【区】【别】【一】【向】【到】【囊】【胚】【晚】【期】【才】【逐】【步】【消】【除】【。】【受】【精】【后】【,】【雌】【性】【胚】【胎】【中】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【同】【步】【进】【行】【敏】【捷】【的】【染】【色】【质】【状】【况】【重】【编】【程】【,】【并】【在】【全】【部】【植】【入】【前】【期】【间】【保】【持】【这】【一】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【之】【间】【染】【色】【质】【状】【况】【的】【精】【确】【平】【衡】【。】【(】【图】【 】【5】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【5】【:】【小】【鼠】【着】【床】【前】【雌】【性】【胚】【胎】【单】【个】【细】【胞】【中】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【的】【动】【态】【改】【变】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【7】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【提】【醒】【了】【小】【鼠】【植】【入】【前】【胚】【胎】【发】【育】【进】【程】【中】【表】【观】【基】【因】【组】【的】【异】【质】【性】【。】【受】【精】【后】【,】【基】【因】【组】【中】【大】【有】【些】【基】【因】【的】【发】【动】【子】【区】【域】【在】【同】【一】【种】【细】【胞】【的】【不】【一】【样】【单】【个】【细】【胞】【之】【间】【保】【持】【着】【均】【匀】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【均】【匀】【的】【染】【色】【质】【敞】【开】【状】【况】【。】【有】【些】【基】【因】【发】【动】【子】【区】【域】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【或】【染】【色】【质】【状】【况】【在】【同】【一】【个】【期】【间】【胚】【胎】【内】【不】【一】【样】【单】【个】【细】【胞】【之】【间】【具】【有】【激】【烈】【的】【异】【质】【性】【。】【但】【是】【,】【发】【动】【子】【区】【域】【 】【D】【N】【A】【 】【甲】【基】【化】【异】【质】【性】【激】【烈】【的】【基】【因】【和】【染】【色】【质】【状】【况】【异】【质】【性】【激】【烈】【的】【基】【因】【别】【离】【是】【两】【类】【不】【一】【样】【的】【基】【因】【。】【这】【暗】【示】【在】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【的】【进】【程】【中】【,】【染】【色】【质】【状】【况】【异】【质】【性】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【异】【质】【性】【也】【许】【别】【离】【受】【不】【一】【样】【机】【制】【的】【调】【控】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【8】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【将】【细】【胞】【周】【期】【与】【染】【色】【质】【状】【况】【联】【系】【了】【起】【来】【。】【研】【讨】【人】【员】【在】【每】【个】【单】【细】【胞】【文】【库】【中】【加】【入】【了】【等】【量】【的】【 】【l】【a】【m】【b】【d】【a】【 】【D】【N】【A】【,】【经】【过】【基】【因】【组】【测】【序】【读】【数】【和】【 】【l】【a】【m】【b】【d】【a】【 】【D】【N】【A】【 】【测】【序】【读】【数】【之】【间】【的】【份】【额】【精】【确】【推】【断】【出】【每】【个】【单】【细】【胞】【的】【倍】【性】【和】【细】【胞】【周】【期】【期】【间】【。】【该】【研】【讨】【运】【用】【小】【鼠】【胚】【胎】【干】【细】【胞】【研】【讨】【中】【已】【有】【的】【染】【色】【质】【优】【先】【仿】【制】【和】【后】【续】【仿】【制】【区】【域】【作】【为】【参】【照】【,】【发】【现】【这】【些】【区】【域】【在】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【仿】【制】【先】【后】【顺】【序】【和】【胚】【胎】【干】【细】【胞】【中】【一】【起】【,】【阐】【明】【小】【鼠】【着】【床】【前】【胚】【胎】【在】【体】【内】【发】【育】【进】【程】【中】【和】【胚】【胎】【干】【细】【胞】【运】【用】【了】【根】【本】【一】【样】【的】【一】【组】【 】【D】【N】【A】【 】【仿】【制】【开】【始】【位】【点】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【6】【:】【 】【小】【鼠】【着】【床】【前】【胚】【胎】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【重】【塑】【特】【征】【示】【意】【图】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【该】【研】【讨】【在】【国】【际】【上】【首】【先】【开】【发】【了】【对】【同】【一】【个】【单】【细】【胞】【能】【够】【一】【起】【研】【讨】【其】【染】【色】【质】【状】【况】【、】【核】【小】【体】【定】【位】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【、】【以】【及】【染】【色】【体】【倍】【性】【 】【5】【 】【个】【层】【面】【的】【单】【细】【胞】【多】【组】【学】【测】【序】【技】【能】【,】【并】【初】【次】【运】【用】【该】【技】【能】【对】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【的】【 】【7】【 】【个】【要】【害】【期】【间】【进】【行】【了】【全】【基】【因】【组】【水】【平】【、】【单】【细】【胞】【分】【辨】【率】【、】【单】【碱】【基】【精】【度】【的】【表】【观】【基】【因】【组】【研】【讨】【,】【并】【深】【度】【解】【析】【了】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【在】【着】【床】【前】【胚】【胎】【发】【育】【中】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【的】【重】【编】【程】【进】【程】【。】【该】【研】【讨】【体】【系】【地】【描】【写】【了】【高】【度】【特】【化】【的】【配】【子】【在】【受】【精】【后】【重】【编】【程】【到】【具】【有】【发】【育】【全】【能】【性】【的】【受】【精】【卵】【、】【以】【及】【进】【一】【步】【发】【育】【成】【多】【能】【性】【胚】【胎】【的】【进】【程】【中】【,】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【发】【作】【的】【精】【准】【、】【有】【序】【的】【改】【变】【,】【以】【及】【各】【个】【组】【学】【层】【面】【之】【间】【的】【互】【动】【联】【系】【(】【图】【 】【6】【)】【。】【该】【工】【作】【为】【往】【后】【大】【家】【持】【续】【研】【讨】【哺】【乳】【动】【物】【前】【期】【胚】【胎】【细】【胞】【全】【能】【性】【和】【多】【能】【性】【的】【敞】【开】【奠】【定】【了】【根】【底】【,】【一】【起】【为】【体】【细】【胞】【克】【隆】【功】【率】【的】【进】【步】【以】【及】【前】【期】【胚】【胎】【发】【育】【反】【常】【的】【确】【诊】【与】【医】【治】【供】【给】【了】【新】【思】【路】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【北】【京】【大】【学】【生】【命】【科】【学】【学】【院】【 】【B】【I】【O】【P】【I】【C】【 】【基】【地】【的】【博】【士】【后】【郭】【帆】【博】【士】【(】【现】【为】【四】【川】【大】【学】【研】【讨】【员】【)】【、】【博】【士】【生】【李】【琳】【、】【李】【静】【云】【为】【该】【论】【文】【的】【并】【列】【第】【一】【作】【者】【;】【北】【京】【大】【学】【生】【命】【科】【学】【学】【院】【汤】【富】【酬】【研】【讨】【员】【和】【四】【川】【大】【学】【郭】【帆】【研】【讨】【员】【为】【这】【篇】【文】【章】【的】【一】【起】【通】【讯】【作】【者】【。】【该】【研】【讨】【工】【作】【由】【北】【京】【大】【学】【和】【四】【川】【大】【学】【一】【起】【协】【作】【完】【结】【,】【而】【且】【得】【到】【了】【国】【家】【自】【然】【科】【学】【基】【金】【委】【员】【会】【、】【北】【京】【将】【来】【基】【因】【确】【诊】【高】【精】【尖】【立】【异】【基】【地】【,】【以】【及】【北】【大】【 】【-】【 】【清】【华】【联】【合】【基】【地】【的】【赞】【助】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【>】【<】【b】【r】【/】【>】【<】【/】【p】【>】【<】【/】【p】【>】【记】【者】【从】【贵】【州】【省】【清】【洗】【计】【生】【委】【发】【布】【的】【最】【新】【消】【息】【得】【悉】【:】【6】【月】【1】【9】【日】【,】【贵】【州】【医】【科】【大】【学】【隶】【属】【白】【云】【分】【院】【陈】【述】【1】【例】【不】【明】【因】【素】【肺】【炎】【病】【例】【,】【贵】【阳】【市】【疾】【控】【中】【心】【标】【本】【检】【查】【成】【果】【为】【H】【7】【N】【9】【核】【酸】【阳】【性】【,】【经】【省】【疾】【控】【中】【心】【流】【感】【实】【验】【室】【复】【核】【和】【临】【床】【专】【家】【确】【诊】【,】【确】【诊】【为】【人】【传】【染】【H】【7】【N】【9】【流】【感】【病】【例】【。】

凯发668k8开户登入

【<】【/】【t】【d】【>】【<】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【6】【p】【x】【;】【"】【>】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【a】【l】【i】【g】【n】【:】【 】【c】【e】【n】【t】【e】【r】【"】【>】【<】【i】【m】【g】【 】【s】【r】【c】【=】【"】【/】【u】【p】【l】【o】【a】【d】【s】【/】【a】【l】【l】【i】【m】【g】【/】【1】【7】【0】【6】【2】【3】【/】【2】【0】【1】【7】【0】【6】【2】【3】【0】【5】【2】【3】【1】【1】【u】【e】【a】【k】【g】【u】【p】【z】【z】【g】【i】【2】【1】【7】【9】【.】【j】【p】【g】【"】【 】【t】【i】【t】【l】【e】【=】【"】【1】【4】【9】【8】【1】【2】【0】【0】【7】【6】【6】【1】【9】【0】【8】【0】【.】【j】【p】【g】【"】【 】【a】【l】【t】【=】【"】【1】【.】【j】【p】【g】【"】【/】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【6】【p】【x】【;】【"】【>】【本】【地】【时】【间】【6】【月】【1】【9】【日】【晚】【,】【2】【0】【1】【7】【我】【国】【深】【圳】【坪】【山】【区】【生】【物】【工】【业】【推】【介】【沟】【通】【会】【在】【美】【国】【圣】【地】【亚】【哥】【举】【办】【,】【坪】【山】【区】【及】【相】【关】【公】【司】【在】【推】【介】【会】【上】【达】【到】【多】【项】【意】【向】【性】【协】【作】【效】【果】【。】【据】【了】【解】【,】【本】【次】【推】【介】【沟】【通】【会】【系】【坪】【山】【区】【生】【物】【公】【司】【沟】【通】【考】【察】【团】【出】【行】【美】【国】【和】【加】【拿】【大】【的】【第】【一】【站】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【6】【p】【x】【;】【"】【>】【 】【 】【 】【 】【坪】【山】【区】【生】【物】【公】【司】【沟】【通】【考】【察】【团】【由】【坪】【山】【区】【委】【书】【记】【吕】【玉】【印】【率】【队】【,】【考】【察】【团】【包】【含】【万】【乐】【药】【业】【、】【华】【因】【康】【基】【因】【、】【理】【邦】【精】【密】【仪】【器】【等】【生】【物】【公】【司】【。】【圣】【地】【亚】【哥】【市】【国】【际】【事】【务】【理】【事】【会】【主】【席】【布】【鲁】【斯】【·】【阿】【巴】【拉】【马】【斯】【以】【及】【美】【国】【本】【地】【近】【1】【0】【0】【家】【生】【物】【公】【司】【的】【董】【事】【长】【、】【总】【裁】【或】【公】【司】【负】【责】【人】【参】【与】【这】【次】【沟】【通】【会】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【6】【p】【x】【;】【"】【>】【 】【 】【 】【 】【据】【了】【解】【,】【广】【东】【省】【与】【加】【利】【福】【尼】【亚】【州】【早】【在】【2】【0】【1】【3】【年】【就】【签】【署】【了】【《】【开】【展】【友】【爱】【沟】【通】【与】【协】【作】【关】【系】【备】【忘】【录】【》】【。】【深】【圳】【和】【圣】【地】【亚】【哥】【都】【是】【诱】【人】【的】【海】【边】【城】【市】【,】【都】【充】【满】【着】【开】【荒】【、】【热】【情】【、】【愿】【望】【、】【创】【业】【的】【空】【气】【,】【也】【都】【高】【度】【重】【视】【生】【物】【工】【业】【的】【开】【展】【。】【圣】【地】【亚】【哥】【具】【有】【在】【全】【球】【生】【物】【范】【畴】【享】【有】【盛】【名】【的】【加】【州】【大】【学】【圣】【地】【亚】【哥】【分】【校】【,】【是】【全】【美】【甚】【至】【国】【际】【生】【物】【医】【药】【工】【业】【的】【主】【要】【聚】【集】【地】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【6】【p】【x】【;】【"】【>】【 】【 】【 】【 】【生】【物】【医】【药】【工】【业】【已】【成】【为】【坪】【山】【区】【主】【要】【的】【支】【柱】【型】【工】【业】【,】【将】【来】【生】【物】【医】【药】【工】【业】【将】【是】【坪】【山】【区】【要】【点】【创】【造】【的】【三】【大】【千】【亿】【级】【工】【业】【集】【群】【之】【一】【。】【数】【据】【显】【现】【,】【当】【时】【坪】【山】【区】【生】【物】【医】【药】【公】【司】【已】【达】【1】【2】【0】【余】【家】【,】【其】【间】【上】【市】【公】【司】【6】【家】【,】【2】【0】【1】【6】【年】【坪】【山】【区】【生】【物】【工】【业】【产】【量】【达】【1】【2】【0】【亿】【元】【人】【民】【币】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【6】【p】【x】【;】【"】【>】【 】【 】【 】【 】【吕】【玉】【印】【在】【推】【介】【会】【上】【表】【明】【,】【坪】【山】【具】【有】【深】【圳】【仅】【有】【的】【国】【家】【级】【生】【物】【工】【业】【基】【地】【。】【该】【基】【地】【计】【划】【面】【积】【3】【.】【3】【平】【方】【公】【里】【,】【集】【聚】【了】【1】【2】【0】【多】【家】【生】【物】【医】【药】【、】【医】【疗】【器】【械】【公】【司】【;】【引】【进】【了】【我】【国】【基】【因】【测】【序】【技】【能】【与】【工】【业】【联】【盟】【、】【广】【东】【医】【疗】【器】【械】【质】【检】【所】【坪】【山】【查】【验】【基】【地】【等】【立】【异】【渠】【道】【。】【期】【望】【坪】【山】【与】【本】【地】【政】【府】【机】【构】【、】【公】【司】【、】【商】【会】【、】【协】【会】【加】【强】【沟】【通】【沟】【通】【,】【树】【立】【长】【时】【间】【严】【密】【的】【协】【作】【沟】【通】【机】【制】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【>】【<】【b】【r】【/】【>】【<】【/】【p】【>】【<】【/】【p】【>】

【<】【t】【d】【 】【r】【o】【w】【s】【p】【a】【n】【=】【"】【3】【"】【>】【贫】【穷】【专】【项】【方】【案】【<】【/】【t】【d】【>】【<】【t】【d】【 】【s】【t】【y】【l】【e】【=】【"】【b】【o】【r】【d】【e】【r】【-】【s】【t】【y】【l】【e】【:】【 】【s】【o】【l】【i】【d】【;】【 】【b】【o】【r】【d】【e】【r】【-】【c】【o】【l】【o】【r】【:】【 】【r】【g】【b】【(】【0】【,】【 】【0】【,】【 】【0】【)】【;】【"】【 】【h】【e】【i】【g】【h】【t】【=】【"】【2】【5】【"】【 】【a】【l】【i】【g】【n】【=】【"】【c】【e】【n】【t】【e】【r】【"】【>】【<】【/】【t】【d】【>】

凯发668k8开户登入到底是什么情况?

【<】【t】【d】【 】【s】【t】【y】【l】【e】【=】【"】【p】【a】【d】【d】【i】【n】【g】【:】【 】【2】【p】【x】【;】【"】【>】【&】【n】【b】【s】【p】【;】【<】【/】【t】【d】【>】【<】【B】【R】【>】【 】【 】【兰】【世】【立】【曾】【对】【外】【表】【明】【,】【2】【0】【0】【7】【年】【他】【与】【王】【石】【、】【冯】【仑】【一】【起】【到】【洪】【山】【监】【狱】【探】【望】【因】【信】【用】【状】【诈】【骗】【案】【入】【狱】【的】【南】【德】【集】【团】【创】【办】【人】【牟】【其】【间】【。】【然】【后】【,】【在】【兰】【世】【立】【因】【躲】【避】【追】【缴】【欠】【税】【罪】【服】【刑】【时】【期】【,】【王】【石】【也】【曾】【去】【狱】【中】【探】【望】【他】【。】【<】【p】【>】【<】【s】【t】【r】【o】【n】【g】【>】【t】【a】【o】【b】【a】【o】【店】【&】【l】【d】【q】【u】【o】【;】【t】【a】【n】【g】【r】【o】【u】【l】【o】【u】【&】【r】【d】【q】【u】【o】【;】【<】【/】【s】【t】【r】【o】【n】【g】【>】【<】【/】【p】【>】

20200410,【<】【/】【p】【>】【<】【/】【t】【b】【o】【d】【y】【>】【 】【 】【本】【案】【中】【,】【A】【房】【子】【挂】【号】【的】【权】【力】【人】【大】【哥】【在】【上】【一】【年】【逝】【世】【后】【,】【原】【告】【赵】【先】【生】【在】【本】【年】【提】【起】【建】【议】【产】【权】【共】【有】【的】【诉】【讼】【,】【是】【在】【诉】【讼】【时】【效】【内】【的】【,】【契】【合】【法】【令】【规】【定】【,】【不】【存】【在】【被】【告】【所】【谓】【的】【超】【越】【诉】【讼】【时】【效】【。】【<】【/】【p】【>】

【<】【p】【 】【c】【l】【a】【s】【s】【=】【"】【M】【s】【o】【N】【o】【r】【m】【a】【l】【"】【 】【a】【l】【i】【g】【n】【=】【"】【r】【i】【g】【h】【t】【"】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【a】【l】【i】【g】【n】【:】【 】【r】【i】【g】【h】【t】【;】【 】【l】【i】【n】【e】【-】【h】【e】【i】【g】【h】【t】【:】【 】【1】【8】【p】【x】【;】【 】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【0】【p】【t】【;】【 】【m】【a】【r】【g】【i】【n】【:】【 】【0】【p】【t】【;】【"】【>】【&】【n】【b】【s】【p】【;】【<】【/】【p】【>】【&】【n】【b】【s】【p】【;】【 】【&】【n】【b】【s】【p】【;】【除】【此】【之】【外】【,】【在】【2】【0】【1】【6】【年】【5】【月】【1】【1】【日】【下】【午】【,】【群】【众】【官】【方】【也】【正】【式】【发】【布】【了】【关】【于】【全】【部】【召】【回】【作】【业】【的】【阶】【段】【性】【通】【报】【。】【在】【通】【报】【中】【指】【出】【:】【&】【l】【d】【q】【u】【o】【;】【通】【过】【几】【个】【月】【紧】【锣】【密】【鼓】【的】【作】【业】【,】【咱】【们】【已】【关】【于】【我】【国】【市】【场】【需】【求】【开】【端】【开】【宣】【布】【一】【套】【具】【有】【监】【测】【和】【预】【警】【功】【用】【的】【召】【回】【补】【救】【办】【法】【,】【该】【专】【属】【办】【法】【已】【根】【本】【完】【成】【验】【证】【作】【业】【。】【本】【着】【对】【用】【户】【高】【度】【负】【责】【的】【谨】【慎】【情】【绪】【,】【为】【保】【证】【该】【办】【法】【在】【各】【种】【路】【况】【下】【的】【适】【用】【性】【和】【可】【靠】【性】【,】【咱】【们】【还】【将】【对】【其】【进】【行】【进】【一】【步】【的】【测】【验】【证】【明】【,】【完】【成】【后】【将】【依】【照】【《】【缺】【点】【轿】【车】【产】【品】【召】【回】【管】【理】【条】【例】【施】【行】【办】【法】【》】【的】【有】【关】【请】【求】【向】【国】【家】【质】【检】【总】【局】【存】【案】【,】【并】【赶】【快】【安】【排】【施】【行】【。】【&】【r】【d】【q】【u】【o】【;】【<】【b】【r】【 】【/】【>】【<】【p】【>】【 】【 】【省】【教】【学】【考】【试】【院】【提】【示】【,】【考】【生】【对】【这】【些】【疑】【问】【应】【分】【外】【留】【意】【。】【不】【管】【哪】【种】【过】【错】【,】【都】【有】【也】【许】【影】【响】【考】【生】【的】【正】【常】【选】【取】【,】【主】【张】【考】【生】【对】【照】【自】【愿】【草】【表】【逐】【个】【批】【次】【、】【逐】【个】【院】【校】【和】【专】【业】【自】【愿】【细】【心】【核】【对】【,】【发】【现】【疑】【问】【及】【时】【纠】【正】【,】【避】【免】【影】【响】【选】【取】【。】【<】【/】【p】【>】

【<】【t】【d】【 】【s】【t】【y】【l】【e】【=】【"】【p】【a】【d】【d】【i】【n】【g】【-】【b】【o】【t】【t】【o】【m】【:】【 】【0】【p】【x】【;】【 】【p】【a】【d】【d】【i】【n】【g】【-】【t】【o】【p】【:】【 】【0】【p】【x】【;】【 】【p】【a】【d】【d】【i】【n】【g】【-】【l】【e】【f】【t】【:】【 】【0】【p】【x】【;】【 】【m】【a】【r】【g】【i】【n】【:】【 】【0】【p】【x】【;】【 】【p】【a】【d】【d】【i】【n】【g】【-】【r】【i】【g】【h】【t】【:】【 】【0】【p】【x】【"】【>】【-】【<】【/】【t】【d】【>】【<】【p】【>】【 】【 】【(】【二】【)】【考】【生】【必】【须】【在】【填】【写】【截】【止】【时】【刻】【前】【完】【结】【网】【上】【填】【写】【自】【愿】【,】【并】【自】【觉】【在】【承】【认】【截】【止】【时】【刻】【前】【到】【承】【认】【点】【进】【行】【承】【认】【。】【抵】【达】【承】【认】【截】【止】【时】【刻】【时】【,】【承】【认】【点】【要】【完】【结】【数】【据】【提】【交】【作】【业】【。】【一】【经】【提】【交】【,】【任】【何】【人】【(】【包】【含】【考】【生】【自】【个】【)】【不】【得】【修】【正】【考】【生】【自】【愿】【信】【息】【。】【<】【/】【p】【>】【<】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【2】【0】【1】【7】【 】【年】【 】【6】【 】【月】【 】【1】【6】【 】【日】【,】【北】【京】【大】【学】【生】【命】【科】【学】【学】【院】【生】【物】【动】【态】【光】【学】【成】【像】【基】【地】【汤】【富】【酬】【课】【题】【组】【在】【《】【C】【e】【l】【l】【 】【R】【e】【s】【e】【a】【r】【c】【h】【》】【杂】【志】【在】【线】【宣】【布】【了】【题】【为】【 】【“】【S】【i】【n】【g】【l】【e】【-】【c】【e】【l】【l】【 】【m】【u】【l】【t】【i】【-】【o】【m】【i】【c】【s】【 】【s】【e】【q】【u】【e】【n】【c】【i】【n】【g】【 】【o】【f】【 】【m】【o】【u】【s】【e】【 】【e】【a】【r】【l】【y】【 】【e】【m】【b】【r】【y】【o】【s】【 】【a】【n】【d】【 】【e】【m】【b】【r】【y】【o】【n】【i】【c】【 】【s】【t】【e】【m】【 】【c】【e】【l】【l】【s】【”】【 】【的】【研】【讨】【论】【文】【。】【在】【国】【际】【上】【首】【先】【开】【展】【了】【对】【一】【个】【单】【细】【胞】【一】【起】【进】【行】【染】【色】【质】【状】【况】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【、】【以】【及】【染】【色】【体】【倍】【性】【的】【全】【基】【因】【组】【测】【序】【技】【能】【(】【s】【i】【n】【g】【l】【e】【-】【c】【e】【l】【l】【 】【C】【O】【O】【L】【-】【s】【e】【q】【)】【,】【并】【选】【用】【这】【一】【技】【能】【在】【单】【细】【胞】【分】【辨】【率】【上】【体】【系】【、】【深】【化】【地】【解】【析】【了】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【表】【观】【基】【因】【组】【重】【编】【程】【的】【要】【害】【特】【征】【,】【以】【及】【染】【色】【质】【状】【况】【与】【 】【D】【N】【A】【 】【甲】【基】【化】【之】【间】【的】【互】【动】【联】【系】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【现】【有】【的】【依】【据】【高】【通】【量】【测】【序】【来】【剖】【析】【全】【基】【因】【组】【染】【色】【质】【状】【况】【的】【研】【讨】【办】【法】【通】【常】【需】【求】【很】【多】【细】【胞】【(】【例】【如】【 】【A】【T】【A】【C】【-】【s】【e】【q】【、】【D】【N】【a】【s】【e】【-】【s】【e】【q】【、】【F】【A】【I】【R】【E】【-】【s】【e】【q】【、】【M】【N】【a】【s】【e】【-】【s】【e】【q】【 】【等】【)】【。】【即】【便】【这】【些】【办】【法】【能】【够】【做】【到】【单】【细】【胞】【分】【辨】【率】【,】【也】【无】【法】【对】【同】【一】【个】【单】【细】【胞】【的】【各】【个】【表】【观】【基】【因】【组】【层】【面】【一】【起】【进】【行】【剖】【析】【,】【因】【此】【无】【法】【在】【单】【细】【胞】【分】【辨】【率】【上】【对】【多】【种】【组】【学】【之】【间】【的】【互】【动】【联】【系】【进】【行】【研】【讨】【。】【而】【汤】【富】【酬】【课】【题】【组】【将】【 】【N】【O】【M】【e】【-】【s】【e】【q】【(】【全】【基】【因】【组】【核】【小】【体】【定】【位】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【组】【测】【序】【)】【技】【能】【和】【 】【P】【B】【A】【T】【-】【s】【e】【q】【 】【技】【能】【(】【全】【基】【因】【组】【重】【亚】【硫】【酸】【盐】【测】【序】【)】【奇】【妙】【地】【联】【系】【起】【来】【(】【图】【 】【1】【)】【,】【并】【进】【行】【了】【体】【系】【的】【优】【化】【和】【进】【步】【,】【完】【结】【了】【对】【同】【一】【个】【单】【细】【胞】【进】【行】【多】【达】【 】【5】【 】【个】【层】【面】【(】【染】【色】【质】【状】【况】【、】【核】【小】【体】【定】【位】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【和】【染】【色】【体】【倍】【性】【)】【的】【基】【因】【组】【和】【表】【观】【基】【因】【组】【特】【征】【的】【剖】【析】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【1】【:】【 】【s】【i】【n】【g】【l】【e】【-】【c】【e】【l】【l】【 】【C】【O】【O】【L】【-】【s】【e】【q】【 】【原】【理】【图】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【除】【了】【能】【够】【在】【单】【细】【胞】【水】【平】【上】【一】【起】【对】【多】【种】【组】【学】【进】【行】【剖】【析】【,】【与】【现】【有】【的】【剖】【析】【染】【色】【质】【状】【况】【的】【单】【细】【胞】【测】【序】【技】【能】【比】【较】【,】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【技】【能】【还】【具】【有】【如】【下】【长】【处】【:】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【1】【)】【关】【于】【基】【因】【组】【中】【基】【地】【的】【功】【用】【元】【件】【区】【域】【具】【有】【很】【高】【的】【灵】【敏】【度】【和】【掩】【盖】【度】【。】【例】【如】【,】【关】【于】【小】【鼠】【胚】【胎】【干】【细】【胞】【系】【的】【一】【个】【单】【细】【胞】【,】【能】【够】【一】【起】【检】【查】【到】【 】【1】【8】【,】【0】【0】【0】【 】【多】【个】【基】【因】【(】【一】【切】【已】【知】【基】【因】【的】【 】【7】【5】【%】【)】【的】【发】【动】【子】【区】【域】【的】【染】【色】【质】【状】【况】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【,】【 】【也】【能】【够】【一】【起】【检】【查】【到】【 】【1】【1】【,】【0】【0】【0】【 】【多】【个】【 】【C】【p】【G】【 】【岛】【(】【一】【切】【已】【知】【 】【C】【p】【G】【 】【岛】【的】【 】【7】【0】【%】【)】【的】【染】【色】【质】【状】【况】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【;】【这】【为】【剖】【析】【干】【细】【胞】【分】【解】【发】【育】【进】【程】【中】【染】【色】【质】【状】【况】【的】【异】【质】【性】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【的】【异】【质】【性】【供】【给】【了】【强】【壮】【的】【东】【西】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【2】【)】【能】【够】【精】【准】【地】【判】【定】【出】【染】【色】【质】【的】【敞】【开】【状】【况】【和】【封】【闭】【状】【况】【,】【精】【确】【地】【把】【染】【色】【质】【封】【闭】【状】【况】【与】【因】【为】【技】【能】【因】【素】【未】【检】【查】【到】【的】【状】【况】【(】【假】【阴】【性】【)】【区】【别】【开】【来】【。】【现】【有】【的】【别】【的】【单】【细】【胞】【染】【色】【质】【状】【况】【测】【序】【技】【能】【(】【例】【如】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【和】【 】【s】【c】【D】【N】【a】【s】【e】【-】【s】【e】【q】【 】【技】【能】【)】【无】【法】【将】【染】【色】【质】【封】【闭】【状】【况】【和】【未】【检】【查】【到】【的】【状】【况】【(】【假】【阴】【性】【)】【区】【别】【开】【,】【这】【一】【缺】【点】【在】【现】【有】【技】【能】【的】【灵】【敏】【度】【比】【较】【低】【的】【状】【况】【下】【尤】【为】【严】【峻】【。】【而】【 】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【技】【能】【能】【够】【精】【确】【地】【区】【别】【染】【色】【质】【封】【闭】【状】【况】【(】【检】【查】【到】【了】【该】【基】【因】【组】【 】【D】【N】【A】【 】【片】【段】【,】【可】【是】【其】【间】【的】【 】【G】【p】【C】【 】【位】【点】【没】【有】【被】【甲】【基】【化】【)】【和】【因】【为】【技】【能】【因】【素】【未】【检】【查】【到】【的】【状】【况】【(】【没】【有】【检】【查】【到】【该】【基】【因】【组】【 】【D】【N】【A】【 】【片】【段】【)】【。】【这】【么】【对】【一】【种】【细】【胞】【类】【型】【中】【不】【一】【样】【的】【单】【个】【细】【胞】【之】【间】【同】【一】【个】【基】【因】【组】【区】【域】【的】【染】【色】【质】【敞】【开】【状】【况】【和】【封】【闭】【状】【况】【之】【间】【的】【份】【额】【能】【够】【给】【出】【精】【确】【的】【丈】【量】【成】【果】【,】【而】【不】【受】【检】【查】【灵】【敏】【度】【动】【摇】【的】【影】【响】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【3】【)】【能】【够】【对】【同】【一】【个】【 】【D】【N】【A】【 】【单】【分】【子】【一】【起】【取】【得】【其】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【信】【息】【,】【使】【得】【这】【一】【技】【能】【不】【光】【具】【有】【单】【细】【胞】【分】【辨】【率】【,】【乃】【至】【到】【达】【了】【单】【分】【子】【分】【辨】【率】【,】【能】【够】【对】【同】【一】【个】【二】【倍】【体】【细】【胞】【的】【两】【个】【等】【位】【基】【因】【别】【离】【进】【行】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【剖】【析】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【4】【)】【因】【为】【该】【办】【法】【一】【起】【扩】【增】【和】【测】【序】【敞】【开】【的】【和】【封】【闭】【的】【染】【色】【质】【区】【域】【,】【因】【此】【不】【受】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【含】【量】【动】【摇】【的】【影】【响】【。】【在】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【通】【常】【是】【环】【形】【暴】【露】【的】【 】【D】【N】【A】【,】【通】【常】【处】【于】【敞】【开】【状】【况】【。】【现】【有】【的】【别】【的】【单】【细】【胞】【染】【色】【质】【状】【况】【测】【序】【技】【能】【(】【例】【如】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【和】【 】【s】【c】【D】【N】【a】【s】【e】【-】【s】【e】【q】【 】【技】【能】【)】【因】【为】【只】【扩】【增】【和】【测】【序】【敞】【开】【的】【染】【色】【质】【区】【域】【,】【因】【此】【极】【大】【地】【受】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【含】【量】【的】【影】【响】【,】【特】【别】【是】【在】【着】【床】【前】【的】【前】【期】【胚】【胎】【细】【胞】【中】【线】【粒】【体】【 】【D】【N】【A】【 】【的】【含】【量】【是】【一】【般】【细】【胞】【的】【数】【十】【倍】【乃】【至】【数】【百】【倍】【,】【因】【此】【关】【于】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【和】【 】【s】【c】【D】【N】【a】【s】【e】【-】【s】【e】【q】【 】【等】【技】【能】【形】【成】【严】【峻】【影】【响】【。】【而】【 】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【技】【能】【因】【为】【一】【起】【扩】【增】【和】【测】【序】【敞】【开】【的】【和】【封】【闭】【的】【染】【色】【质】【区】【域】【而】【不】【受】【这】【一】【疑】【问】【的】【影】【响】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【5】【)】【因】【为】【掺】【入】【了】【 】【1】【0】【%】【 】【-】【 】【2】【0】【%】【 】【的】【外】【源】【 】【l】【a】【m】【b】【d】【a】【 】【D】【N】【A】【,】【所】【以】【能】【够】【精】【确】【判】【别】【所】【剖】【析】【单】【个】【细】【胞】【所】【在】【的】【细】【胞】【周】【期】【期】【间】【以】【及】【染】【色】【体】【倍】【性】【。】【这】【关】【于】【精】【确】【剖】【析】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【与】【细】【胞】【周】【期】【以】【及】【染】【色】【体】【倍】【性】【的】【联】【系】【十】【分】【有】【协】【助】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【考】【虑】【到】【多】【组】【学】【的】【信】【息】【量】【、】【有】【用】【数】【据】【量】【带】【来】【的】【昂】【扬】【测】【序】【本】【钱】【等】【疑】【问】【,】【关】【于】【细】【胞】【数】【量】【少】【,】【异】【质】【性】【强】【的】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【的】【研】【讨】【,】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【办】【法】【十】【分】【合】【适】【。】【该】【办】【法】【能】【够】【非】【常】【好】【地】【掩】【盖】【全】【基】【因】【组】【,】【有】【用】【地】【处】【理】【了】【如】【今】【 】【s】【c】【A】【T】【A】【C】【-】【s】【e】【q】【 】【研】【讨】【中】【线】【粒】【体】【片】【段】【过】【度】【富】【集】【致】【使】【的】【有】【用】【数】【据】【量】【过】【少】【的】【疑】【问】【。】【一】【起】【,】【该】【办】【法】【能】【够】【一】【起】【剖】【析】【单】【个】【细】【胞】【中】【染】【色】【质】【敞】【开】【程】【度】【、】【核】【小】【体】【定】【位】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【、】【以】【及】【染】【色】【体】【倍】【性】【这】【 】【5】【 】【个】【组】【学】【层】【面】【,】【关】【于】【难】【以】【很】【多】【取】【得】【的】【哺】【乳】【动】【物】【前】【期】【胚】【胎】【的】【发】【育】【、】【以】【及】【杂】【乱】【的】【癌】【症】【等】【疾】【病】【研】【讨】【,】【都】【将】【供】【给】【全】【部】【有】【用】【的】【处】【理】【方】【案】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【在】【此】【根】【底】【上】【,】【该】【课】【题】【组】【运】【用】【这】【一】【新】【树】【立】【的】【 】【s】【c】【C】【O】【O】【L】【-】【s】【e】【q】【 】【办】【法】【,】【在】【单】【细】【胞】【分】【辨】【率】【体】【系】【地】【描】【写】【了】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【表】【观】【基】【因】【组】【多】【个】【层】【面】【的】【动】【态】【改】【变】【。】【高】【度】【特】【化】【的】【精】【子】【和】【卵】【细】【胞】【联】【系】【后】【,】【会】【进】【行】【一】【系】【列】【杂】【乱】【、】【精】【确】【的】【染】【色】【质】【重】【塑】【进】【程】【,】【然】【后】【树】【立】【起】【前】【期】【胚】【胎】【发】【育】【的】【全】【能】【性】【和】【多】【能】【性】【。】【对】【该】【进】【程】【的】【解】【析】【将】【有】【利】【于】【大】【家】【了】【解】【细】【胞】【多】【能】【性】【树】【立】【进】【程】【中】【的】【表】【观】【遗】【传】【调】【控】【机】【制】【以】【及】【胚】【胎】【发】【育】【反】【常】【的】【分】【子】【机】【理】【,】【该】【项】【研】【讨】【发】【现】【:】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【1】【)】【受】【精】【后】【 】【1】【2】【 】【小】【时】【以】【内】【,】【来】【自】【高】【度】【特】【化】【的】【卵】【细】【胞】【和】【精】【子】【的】【男】【女】【原】【核】【就】【阅】【历】【了】【大】【规】【模】【的】【基】【因】【组】【去】【甲】【基】【化】【,】【父】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【程】【度】【从】【精】【子】【中】【的】【 】【8】【0】【%】【(】【平】【均】【数】【)】【降】【低】【到】【雄】【原】【核】【中】【的】【 】【3】【8】【%】【(】【p】【=】【1】【.】【4】【×】【1】【0】【-】【1】【1】【)】【;】【一】【起】【母】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【程】【度】【从】【卵】【细】【胞】【中】【的】【 】【3】【2】【%】【 】【降】【低】【到】【雌】【原】【核】【中】【的】【 】【2】【8】【%】【(】【p】【=】【6】【.】【3】【×】【1】【0】【-】【5】【)】【。】【在】【此】【进】【程】【中】【,】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【体】【状】【况】【敏】【捷】【翻】【开】【,】【在】【受】【精】【卵】【的】【原】【核】【期】【就】【现】【已】【到】【达】【高】【度】【敞】【开】【的】【状】【况】【,】【随】【后】【在】【受】【精】【卵】【晚】【期】【染】【色】【质】【敞】【开】【程】【度】【大】【幅】【度】【回】【落】【,】【并】【在】【 】【2】【 】【-】【 】【细】【胞】【期】【间】【以】【后】【敞】【开】【程】【度】【再】【次】【逐】【步】【添】【加】【,】【到】【囊】【胚】【期】【时】【到】【达】【最】【高】【点】【(】【图】【 】【2】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【2】【:】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【基】【因】【组】【内】【源】【 】【D】【N】【A】【 】【甲】【基】【化】【(】【A】【)】【与】【染】【色】【质】【状】【况】【(】【B】【)】【以】【及】【染】【色】【质】【状】【况】【异】【质】【性】【(】【基】【因】【发】【动】【子】【区】【域】【;】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【o】【p】【e】【n】【、】【D】【i】【v】【e】【r】【g】【e】【n】【t】【、】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【c】【l】【o】【s】【e】【d】【 】【三】【种】【异】【质】【性】【状】【况】【)】【(】【C】【)】【(】【D】【)】【的】【动】【态】【改】【变】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【2】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【体】【系】【剖】【析】【了】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【染】【色】【质】【状】【况】【的】【异】【质】【性】【。】【依】【据】【每】【个】【基】【因】【发】【动】【子】【区】【域】【在】【同】【一】【个】【发】【育】【期】【间】【不】【一】【样】【单】【细】【胞】【之】【间】【染】【色】【质】【状】【况】【的】【异】【质】【性】【,】【将】【小】【鼠】【的】【基】【因】【划】【分】【红】【均】【匀】【敞】【开】【(】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【o】【p】【e】【n】【)】【、】【敞】【开】【 】【/】【 】【封】【闭】【混】【合】【态】【(】【D】【i】【v】【e】【r】【g】【e】【n】【t】【)】【、】【均】【匀】【封】【闭】【(】【H】【o】【m】【o】【g】【e】【n】【e】【o】【u】【s】【l】【y】【 】【c】【l】【o】【s】【e】【d】【)】【这】【三】【种】【状】【况】【。】【该】【研】【讨】【发】【如】【今】【受】【精】【后】【 】【1】【2】【 】【个】【小】【时】【以】【内】【受】【精】【卵】【中】【大】【有】【些】【基】【因】【的】【发】【动】【子】【区】【域】【就】【由】【均】【匀】【封】【闭】【状】【况】【敏】【捷】【重】【编】【程】【为】【均】【匀】【敞】【开】【状】【况】【,】【为】【合】【子】【基】【因】【在】【随】【后】【的】【转】【录】【做】【好】【预】【备】【(】【图】【 】【2】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【3】【)】【经】【过】【 】【R】【N】【A】【 】【聚】【合】【酶】【按】【捺】【剂】【按】【捺】【转】【录】【的】【试】【验】【,】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【证】【实】【持】【续】【转】【录】【关】【于】【保】【持】【基】【因】【发】【动】【子】【区】【域】【的】【染】【色】【质】【敞】【开】【状】【况】【具】【有】【主】【要】【作】【用】【。】【从】【受】【精】【前】【的】【卵】【细】【胞】【到】【受】【精】【后】【的】【晚】【期】【受】【精】【卵】【期】【间】【,】【发】【动】【子】【区】【大】【片】【段】【染】【色】【质】【敞】【开】【区】【域】【(】【长】【度】【大】【于】【 】【3】【0】【0】【b】【p】【)】【的】【数】【量】【大】【幅】【度】【添】【加】【。】【该】【研】【讨】【发】【现】【,】【运】【用】【 】【R】【N】【A】【 】【聚】【合】【酶】【按】【捺】【剂】【α】【-】【A】【m】【a】【n】【i】【t】【i】【n】【 】【处】【理】【受】【精】【卵】【、】【按】【捺】【其】【转】【录】【活】【动】【,】【会】【致】【使】【数】【千】【个】【(】【5】【6】【%】【)】【基】【因】【的】【发】【动】【子】【区】【大】【片】【段】【染】【色】【质】【敞】【开】【区】【域】【从】【头】【封】【闭】【,】【阐】【明】【持】【续】【的】【转】【录】【关】【于】【保】【持】【前】【期】【胚】【胎】【中】【大】【有】【些】【基】【因】【的】【发】【动】【子】【处】【于】【敞】【开】【状】【况】【是】【必】【需】【的】【,】【染】【色】【质】【状】【况】【敞】【开】【和】【转】【录】【活】【动】【互】【相】【促】【进】【,】【一】【起】【保】【持】【合】【子】【基】【因】【的】【安】【稳】【表】【达】【(】【图】【 】【3】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【4】【)】【发】【现】【多】【能】【性】【基】【地】【因】【子】【 】【O】【c】【t】【4】【 】【的】【靶】【基】【因】【联】【系】【位】【点】【在】【 】【4】【 】【-】【 】【细】【胞】【期】【间】【就】【现】【已】【翻】【开】【并】【处】【于】【敞】【开】【状】【况】【,】【远】【早】【于】【真】【实】【树】【立】【多】【能】【性】【的】【囊】【胚】【期】【,】【暗】【示】【这】【些】【位】【点】【作】【为】【潜】【在】【的】【顺】【式】【调】【控】【元】【件】【也】【许】【参】【加】【了】【前】【期】【胚】【胎】【细】【胞】【的】【命】【运】【决】【议】【进】【程】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【5】【)】【初】【次】【在】【单】【个】【细】【胞】【内】【对】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【以】【及】【 】【D】【N】【A】【 】【甲】【基】【化】【进】【行】【了】【深】【化】【剖】【析】【。】【从】【受】【精】【卵】【晚】【期】【到】【 】【4】【 】【细】【胞】【胚】【胎】【期】【间】【,】【在】【基】【因】【间】【区】【(】【i】【n】【t】【e】【r】【g】【e】【n】【i】【c】【 】【r】【e】【g】【i】【o】【n】【)】【父】【源】【基】【因】【组】【甲】【基】【化】【要】【显】【着】【高】【于】【母】【源】【基】【因】【组】【;】【而】【在】【基】【因】【区】【(】【g】【e】【n】【e】【 】【b】【o】【d】【y】【)】【,】【父】【源】【基】【因】【组】【甲】【基】【化】【要】【显】【着】【低】【于】【母】【源】【基】【因】【组】【。】【这】【是】【因】【为】【在】【基】【因】【间】【区】【,】【父】【源】【基】【因】【组】【的】【去】【甲】【基】【化】【速】【度】【较】【慢】【;】【而】【在】【基】【因】【区】【,】【父】【源】【基】【因】【组】【的】【去】【甲】【基】【化】【速】【度】【要】【远】【快】【于】【母】【源】【基】【因】【组】【的】【去】【甲】【基】【化】【速】【度】【。】【更】【主】【要】【的】【是】【,】【关】【于】【基】【因】【区】【,】【胚】【胎】【期】【表】【达】【水】【平】【越】【高】【的】【基】【因】【其】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【不】【对】【称】【性】【越】【激】【烈】【(】【表】【达】【水】【平】【越】【高】【,】【母】【源】【基】【因】【组】【甲】【基】【化】【就】【比】【父】【源】【基】【因】【组】【高】【越】【多】【)】【。】【与】【此】【相】【反】【,】【受】【精】【后】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【就】【敏】【捷】【同】【步】【翻】【开】【,】【到】【受】【精】【后】【 】【1】【2】【 】【小】【时】【,】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【在】【每】【个】【单】【细】【胞】【中】【就】【现】【已】【到】【达】【一】【样】【的】【敞】【开】【状】【况】【,】【并】【在】【全】【部】【植】【入】【前】【期】【间】【保】【持】【这】【一】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【之】【间】【染】【色】【质】【状】【况】【的】【精】【确】【平】【衡】【。】【这】【阐】【明】【受】【精】【后】【,】【染】【色】【质】【状】【况】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【进】【行】【了】【不】【一】【样】【步】【的】【重】【编】【程】【进】【程】【,】【因】【为】【染】【色】【质】【状】【况】【关】【于】【合】【子】【基】【因】【的】【激】【活】【也】【许】【更】【主】【要】【,】【所】【以】【受】【精】【后】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【的】【染】【色】【质】【状】【况】【敏】【捷】【重】【编】【程】【、】【在】【每】【个】【单】【细】【胞】【中】【敏】【捷】【到】【达】【精】【确】【平】【衡】【并】【一】【向】【保】【持】【。】【而】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【重】【编】【程】【要】【慢】【一】【些】【并】【在】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【之】【间】【保】【持】【不】【对】【称】【散】【布】【:】【在】【基】【因】【间】【区】【,】【父】【源】【基】【因】【组】【甲】【基】【化】【高】【于】【母】【源】【基】【因】【组】【;】【而】【在】【基】【因】【区】【,】【父】【源】【基】【因】【组】【甲】【基】【化】【低】【于】【母】【源】【基】【因】【组】【,】【而】【且】【与】【胚】【胎】【期】【基】【因】【表】【达】【水】【平】【有】【关】【(】【图】【 】【4】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【4】【:】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【不】【对】【称】【散】【布】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【6】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【解】【析】【了】【雌】【性】【胚】【胎】【细】【胞】【中】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【重】【编】【程】【进】【程】【的】【异】【同】【。】【从】【配】【子】【到】【原】【核】【期】【受】【精】【卵】【,】【父】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【去】【甲】【基】【化】【速】【度】【显】【着】【比】【常】【染】【色】【体】【慢】【,】【而】【母】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【去】【甲】【基】【化】【速】【度】【和】【常】【染】【色】【体】【适】【当】【。】【从】【受】【精】【卵】【晚】【期】【到】【 】【4】【 】【细】【胞】【胚】【胎】【期】【间】【,】【父】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【显】【着】【高】【于】【母】【源】【 】【X】【 】【染】【色】【体】【,】【直】【到】【囊】【胚】【期】【,】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【水】【平】【才】【到】【达】【一】【起】【。】【这】【阐】【明】【受】【精】【以】【后】【,】【在】【雌】【性】【胚】【胎】【中】【失】【活】【的】【父】【源】【 】【X】【 】【染】【色】【体】【其】【 】【D】【N】【A】【 】【甲】【基】【化】【重】【编】【程】【速】【度】【要】【显】【着】【慢】【于】【活】【泼】【的】【母】【源】【 】【X】【 】【染】【色】【体】【。】【二】【者】【之】【间】【 】【D】【N】【A】【 】【甲】【基】【化】【的】【区】【别】【一】【向】【到】【囊】【胚】【晚】【期】【才】【逐】【步】【消】【除】【。】【受】【精】【后】【,】【雌】【性】【胚】【胎】【中】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【同】【步】【进】【行】【敏】【捷】【的】【染】【色】【质】【状】【况】【重】【编】【程】【,】【并】【在】【全】【部】【植】【入】【前】【期】【间】【保】【持】【这】【一】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【之】【间】【染】【色】【质】【状】【况】【的】【精】【确】【平】【衡】【。】【(】【图】【 】【5】【)】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【5】【:】【小】【鼠】【着】【床】【前】【雌】【性】【胚】【胎】【单】【个】【细】【胞】【中】【爸】【爸】【妈】【妈】【源】【 】【X】【 】【染】【色】【体】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【的】【动】【态】【改】【变】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【7】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【提】【醒】【了】【小】【鼠】【植】【入】【前】【胚】【胎】【发】【育】【进】【程】【中】【表】【观】【基】【因】【组】【的】【异】【质】【性】【。】【受】【精】【后】【,】【基】【因】【组】【中】【大】【有】【些】【基】【因】【的】【发】【动】【子】【区】【域】【在】【同】【一】【种】【细】【胞】【的】【不】【一】【样】【单】【个】【细】【胞】【之】【间】【保】【持】【着】【均】【匀】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【均】【匀】【的】【染】【色】【质】【敞】【开】【状】【况】【。】【有】【些】【基】【因】【发】【动】【子】【区】【域】【的】【 】【D】【N】【A】【 】【甲】【基】【化】【或】【染】【色】【质】【状】【况】【在】【同】【一】【个】【期】【间】【胚】【胎】【内】【不】【一】【样】【单】【个】【细】【胞】【之】【间】【具】【有】【激】【烈】【的】【异】【质】【性】【。】【但】【是】【,】【发】【动】【子】【区】【域】【 】【D】【N】【A】【 】【甲】【基】【化】【异】【质】【性】【激】【烈】【的】【基】【因】【和】【染】【色】【质】【状】【况】【异】【质】【性】【激】【烈】【的】【基】【因】【别】【离】【是】【两】【类】【不】【一】【样】【的】【基】【因】【。】【这】【暗】【示】【在】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【的】【进】【程】【中】【,】【染】【色】【质】【状】【况】【异】【质】【性】【和】【 】【D】【N】【A】【 】【甲】【基】【化】【异】【质】【性】【也】【许】【别】【离】【受】【不】【一】【样】【机】【制】【的】【调】【控】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【(】【8】【)】【初】【次】【在】【单】【细】【胞】【分】【辨】【率】【将】【细】【胞】【周】【期】【与】【染】【色】【质】【状】【况】【联】【系】【了】【起】【来】【。】【研】【讨】【人】【员】【在】【每】【个】【单】【细】【胞】【文】【库】【中】【加】【入】【了】【等】【量】【的】【 】【l】【a】【m】【b】【d】【a】【 】【D】【N】【A】【,】【经】【过】【基】【因】【组】【测】【序】【读】【数】【和】【 】【l】【a】【m】【b】【d】【a】【 】【D】【N】【A】【 】【测】【序】【读】【数】【之】【间】【的】【份】【额】【精】【确】【推】【断】【出】【每】【个】【单】【细】【胞】【的】【倍】【性】【和】【细】【胞】【周】【期】【期】【间】【。】【该】【研】【讨】【运】【用】【小】【鼠】【胚】【胎】【干】【细】【胞】【研】【讨】【中】【已】【有】【的】【染】【色】【质】【优】【先】【仿】【制】【和】【后】【续】【仿】【制】【区】【域】【作】【为】【参】【照】【,】【发】【现】【这】【些】【区】【域】【在】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【进】【程】【中】【仿】【制】【先】【后】【顺】【序】【和】【胚】【胎】【干】【细】【胞】【中】【一】【起】【,】【阐】【明】【小】【鼠】【着】【床】【前】【胚】【胎】【在】【体】【内】【发】【育】【进】【程】【中】【和】【胚】【胎】【干】【细】【胞】【运】【用】【了】【根】【本】【一】【样】【的】【一】【组】【 】【D】【N】【A】【 】【仿】【制】【开】【始】【位】【点】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【图】【 】【6】【:】【 】【小】【鼠】【着】【床】【前】【胚】【胎】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【重】【塑】【特】【征】【示】【意】【图】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【该】【研】【讨】【在】【国】【际】【上】【首】【先】【开】【发】【了】【对】【同】【一】【个】【单】【细】【胞】【能】【够】【一】【起】【研】【讨】【其】【染】【色】【质】【状】【况】【、】【核】【小】【体】【定】【位】【、】【D】【N】【A】【 】【甲】【基】【化】【、】【基】【因】【组】【拷】【贝】【数】【变】【异】【、】【以】【及】【染】【色】【体】【倍】【性】【 】【5】【 】【个】【层】【面】【的】【单】【细】【胞】【多】【组】【学】【测】【序】【技】【能】【,】【并】【初】【次】【运】【用】【该】【技】【能】【对】【小】【鼠】【着】【床】【前】【胚】【胎】【发】【育】【的】【 】【7】【 】【个】【要】【害】【期】【间】【进】【行】【了】【全】【基】【因】【组】【水】【平】【、】【单】【细】【胞】【分】【辨】【率】【、】【单】【碱】【基】【精】【度】【的】【表】【观】【基】【因】【组】【研】【讨】【,】【并】【深】【度】【解】【析】【了】【爸】【爸】【妈】【妈】【源】【基】【因】【组】【在】【着】【床】【前】【胚】【胎】【发】【育】【中】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【的】【重】【编】【程】【进】【程】【。】【该】【研】【讨】【体】【系】【地】【描】【写】【了】【高】【度】【特】【化】【的】【配】【子】【在】【受】【精】【后】【重】【编】【程】【到】【具】【有】【发】【育】【全】【能】【性】【的】【受】【精】【卵】【、】【以】【及】【进】【一】【步】【发】【育】【成】【多】【能】【性】【胚】【胎】【的】【进】【程】【中】【,】【 】【D】【N】【A】【 】【甲】【基】【化】【和】【染】【色】【质】【状】【况】【发】【作】【的】【精】【准】【、】【有】【序】【的】【改】【变】【,】【以】【及】【各】【个】【组】【学】【层】【面】【之】【间】【的】【互】【动】【联】【系】【(】【图】【 】【6】【)】【。】【该】【工】【作】【为】【往】【后】【大】【家】【持】【续】【研】【讨】【哺】【乳】【动】【物】【前】【期】【胚】【胎】【细】【胞】【全】【能】【性】【和】【多】【能】【性】【的】【敞】【开】【奠】【定】【了】【根】【底】【,】【一】【起】【为】【体】【细】【胞】【克】【隆】【功】【率】【的】【进】【步】【以】【及】【前】【期】【胚】【胎】【发】【育】【反】【常】【的】【确】【诊】【与】【医】【治】【供】【给】【了】【新】【思】【路】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【 】【s】【t】【y】【l】【e】【=】【"】【t】【e】【x】【t】【-】【i】【n】【d】【e】【n】【t】【:】【 】【2】【e】【m】【;】【"】【>】【<】【s】【p】【a】【n】【 】【s】【t】【y】【l】【e】【=】【"】【f】【o】【n】【t】【-】【s】【i】【z】【e】【:】【 】【1】【4】【p】【x】【;】【"】【>】【北】【京】【大】【学】【生】【命】【科】【学】【学】【院】【 】【B】【I】【O】【P】【I】【C】【 】【基】【地】【的】【博】【士】【后】【郭】【帆】【博】【士】【(】【现】【为】【四】【川】【大】【学】【研】【讨】【员】【)】【、】【博】【士】【生】【李】【琳】【、】【李】【静】【云】【为】【该】【论】【文】【的】【并】【列】【第】【一】【作】【者】【;】【北】【京】【大】【学】【生】【命】【科】【学】【学】【院】【汤】【富】【酬】【研】【讨】【员】【和】【四】【川】【大】【学】【郭】【帆】【研】【讨】【员】【为】【这】【篇】【文】【章】【的】【一】【起】【通】【讯】【作】【者】【。】【该】【研】【讨】【工】【作】【由】【北】【京】【大】【学】【和】【四】【川】【大】【学】【一】【起】【协】【作】【完】【结】【,】【而】【且】【得】【到】【了】【国】【家】【自】【然】【科】【学】【基】【金】【委】【员】【会】【、】【北】【京】【将】【来】【基】【因】【确】【诊】【高】【精】【尖】【立】【异】【基】【地】【,】【以】【及】【北】【大】【 】【-】【 】【清】【华】【联】【合】【基】【地】【的】【赞】【助】【。】【<】【/】【s】【p】【a】【n】【>】【<】【/】【p】【>】【<】【p】【>】【<】【b】【r】【/】【>】【<】【/】【p】【>】【<】【/】【p】【>】

乐天堂会员注册

【记】【者】【从】【贵】【州】【省】【清】【洗】【计】【生】【委】【发】【布】【的】【最】【新】【消】【息】【得】【悉】【:】【6】【月】【1】【9】【日】【,】【贵】【州】【医】【科】【大】【学】【隶】【属】【白】【云】【分】【院】【陈】【述】【1】【例】【不】【明】【因】【素】【肺】【炎】【病】【例】【,】【贵】【阳】【市】【疾】【控】【中】【心】【标】【本】【检】【查】【成】【果】【为】【H】【7】【N】【9】【核】【酸】【阳】【性】【,】【经】【省】【疾】【控】【中】【心】【流】【感】【实】【验】【室】【复】【核】【和】【临】【床】【专】【家】【确】【诊】【,】【确】【诊】【为】【人】【传】【染】【H】【7】【N】【9】【流】【感】【病】【例】【。】

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